I am an experienced Postdoc who recently joined the Soft Condensed Matter group to take part in a very interesting, and at the same time socially relevant, project on Nanoplastics in living cells.
My previous scientific experience is as follows:
2019 December- to March 2022, Postdoctoral Research Fellow, Warsaw University of Life Science (SGGW), Warsaw, Poland
Working in projects:
– Sonata Bis, NCN: Structural and functional characterization of SlpA the main S-layer protein of Deinococcus radiodurans;
– Harmonia, NCN: Solving the elaborated architecture of the D. radiodurans S-layer- structural analysis of its type IV-like piliation system;
– Urgency Grants, NAWA: Structural insights into the assembly of the Nucleocapsid protein of SARS-CoV-2.
– working with membrane proteins and membrane protein complexes expression, isolation and biochemical characterization;
– three dimensional and two dimensional crystallization of membrane proteins. Electron crystallography and X-ray crystallography using various X-ray sources;
– IR and UV-Vis spectroscopy on membrane protein complexes which bind chromophores such as chlorophylls, carotenoids, phycobilins and similar.
– Photocurrents Measured from a Monolayer of Adsorbed Reaction Center (RC) Protein on Gold, International Online Conference on Bio-hybrid Approaches to Solar Energy Conversion (Biohybrid), Online, Spain, 2020 October 27th – 29th.
2016- 2019, Postdoctoral Research Fellow, Center of New Technologies, University of Warsaw, Poland
Working in projects:
– OPUS, NCN: Structural and functional characterisation of the photosynthetic apparatus of an extremophilic red microalga Cyanidioschyzon merolae;
– OPUS, NCN: Insight into combined electrochemical-photochemical activation of carbon dioxide;
– PolTur, NCiBR: Graphene-derived electrodes for bio-inspired solar-fuel device;
– SunRise, Founding Partner of H2020 SUNRISE Coordination and Support Action: Solar Energy for a Circular Economy.
– working with microalga Cyanidioschyzon merolae and cyanobacterium Thermosynechococcus elongatus;
– working with photosynthetic apparatus (photosystem I, photosystem II, light harvesting complexes);
– many laboratory techniques (chromatographic approaches (AEC, HIC, IMAC, SEC), biochemical techniques (SDS-PAGE, BN-PAGE, Western blotting, sucrose gradient fractionation etc.), molecular biology methods (RT-PCR, DNA cloning), spectroscopic methods (fluorescence and absorption spectroscopy), photoelectrochemistry and bioinformatics);
– electrochemical-photochemical analysis.
– Four short internships to Center for Free-electron Laser science (CFEL), Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany;
-Two short internship to Diamond Light Source, Oxford, United Kingdom.
– 1st PolTur/GrapESol Workshop on Biohybrid Graphene Solar Cells, 20-22.09.2016,Warsaw, Poland (My Lecture: ‘Preparation of perfect biological material for constructing truly ‘green’ viable biohybrid solar-to-fuel nanodevices’);
-The 17th International Congress on Photosynthesis Research, 7-12.08.2016, Maastricht, Netherlands, (My lecture: ‘Molecular mechanisms of photoprotection in highly robust PSI-LHCI supercomplex from an extremophilic red alga Cyanidioschyzon merolae’).
During this time I have open many collaboration with top national and international experts (Kasim Ocakoglu, Mersin University, Turkey; Roman Kouřil, Palacký University, Olomouc, the Czech Republic; Dwayne Miller, Max Planck Institute for the Structure and Dynamics of Matter, Hamburg, Germany; Sebastian Maćkowski, Nicolaus Copernicus University, Toruń).
2009-2015 PhD student at Nencki Institute of Experimental Biology, however the experimental work performed in Laboratory of Structural Biology, International Institute of Molecular and Cell Biology, Junior research Group (Max Planck Society and Polish Academy of Science) Warsaw (Poland)
Working in projects:
– Marie-Curie Transfer of Knowledge grant MemProt ‘Structural studies of membranę proteases’;
– Homing Plus program, Foundation for Polish Science, co-financed by the European Union under the European Regional Development Funds, Title: ‘Structural and Functional Characterization of Photosystem II from Nicotiana tabacum’;
– Preludium grant NCN, Title: ‘Analysis role of the Psbs subunit from photosystem II in the non-photochemical quenching’.
– working with membrane protein;
– working with photosystem II from higher plants (Nicotiana tabacum, Spinacia oleracea, Ginko biloba);
– in being a Principal Investigator of PRELUDIUM grant (National Science Center);
– in crystallography (obtaining, freezing and soaking crystals of globular and membrane proteins and their complexes, registration of diffraction data using the home-source and synchrotron radiation, diffraction);
– in improving all laboratory skills.
– Two short internship to Diamond Light Source, Oxford, United Kingdom;
– Two short internship to Germany’s 3rd generation synchrotron radiation facilities, BESSY II, Berlin Germany.
Conferences and workshops:
-15th Heart of Europe bioCrystallography Meeting, 28-30.09.2012, Beilngries, Germany;
– BIO 2014 1st Polish Congress of Biochemistry, Cell Biology, Biophysics and Bioinformatics, 9-12.09.2012, Warsaw, Poland;
– International School of Biophysics: Channels and Transporters, 10-18.05.2011, Erice, Italy (winning scholarship);
– 13th Heart of Europe bioCrystallography Meeting, 24-26.09.2010;
– Membrane proteins -production, purification, stabilisation and structures, 15-21.11.2009, Leeds, United Kingdom (winning scholarship);
– Membrane Protein Production, crystallisation and crystallography, 24.06.2009, Diamond, United Kingdom (winning scholarship);
– The 1st Biannual Mykonos Workshop: Membrane Protein Frontiers: Dynamics, Energy, Structure, and Technology, 28.05.-2.06.2009, Mykonos, Greece.