A. Karpińska, M. Pilz, J. Buczkowska, P. J. Żuk, K. Kucharska, G. Magiera, K. Kwapiszewska, and R. Hołyst
Analyst, 2021,146, 7131-7143
Quantitative description of biochemical processes inside living cells and at single-molecule levels remains a challenge at the forefront of modern instrumentation and spectroscopy. This paper demonstrates such single-cell, single-molecule analyses performed to study the mechanism of action of olaparib – an up-to-date, FDA-approved drug for germline-BRCA mutated metastatic breast cancer.
M. Gawrys-Kopczynska, M. Konop, K. Maksymiuk, K. Kraszewska, L. Derzsi, K. Sozanski, R. Holyst, M. Pilz and E. Samborowska
ELIFE 2020, 9
Trimethylamine-oxide (TMAO) is present in seafood which is considered to be beneficial for health. Deep-water animals accumulate TMAO to protect proteins, such as lactate dehydrogenase (LDH), against hydrostatic pressure stress (HPS).
Pilz, Marta and Kwapiszewska, Karina and Kalwarczyk, Tomasz and Bubak, Grzegorz and Nowis, Dominika and Holyst, Robertv
The Royal Society of Chemistry, Nanoscale, 2020,12, 19880-19887
The efficient delivery of drugs to cells depends on their diffusion through the extracellular matrix (ECM) of tissues. Here we present a study of diffusion of nanoprobes of radius from 1 nm to over 100 nm in the ECM of spheroids of three cell types (HeLa, MCF-7 and fibroblasts).
K. Jaworska, D. Hering , G. Mosieniak, A. Bielak-Zmijewska, M. Pilz, M. Konwerski, A. Gasecka, A. Kapłon-Cieślicka, K. Filipiak, E. Sikora, R. Hołyst and M. Ufnal
Toxins 2019, 11(9), 490
Trimethylamine-N-oxide (TMAO) has been suggested as a marker and mediator of cardiovascular diseases. However, data are contradictory, and the mechanisms are obscure. Strikingly, the role of the TMAO precursor trimethylamine (TMA) has not drawn attention in cardiovascular studies even though toxic effects of TMA were proposed several decades ago.